alpha300 R – Raman Imaging Microscope
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alpha300 R – Raman Imaging Microscope
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alpha300 R – Raman Imaging Microscope

Benefits of the alpha300 R series

• Identification and visualization of the distribution of chemical compounds

• Analysis of crystallinity and material stress properties

• Lateral, diffraction-limited spatial resolution down to ~200 nm

• 3D chemical imaging enabled by confocal architecture

• Nondestructive

• No staining or labelling necessary

• Correlative imaging options readily available (i.e. fluorescence microscopy, AFM, SNOM, SEM, TERS)

Pioneering techniques, maximum versatility and ease of operation – that is what WITec, the Raman imaging company, stands for. To meet the most exacting requirements of our customers, WITec continually develops microscopy systems for correlative Raman imaging that fulfill three crucial tasks simultaneously: they provide the best spatial and spectral resolution along with peerless sensitivity and unmatched speed.

Ultrahigh-throughput UHTS Spectrometers

  • Various lens-based, excitation optimized spectrometers (UV, VIS or NIR) available, all specifically designed for Raman microsopy and applications with low light intensities

  • Fibre-coupled ultrahigh-throughput optical instruments
  • Superior peak shape conservation

 

 

Basic Microscope Features

  • Research grade optical microscope with 6x objective turret

  • Video system: video CCD camera

  • LED white-light source for Köhler illumination

  • Manual sample positioning in x- and y-direction

  • Fibre coupling

Key Features

  • Confocal Raman Imaging with unprecedented performance in speed, sensitivity, and resolution

  • Hyperspectral image generation with the information of a complete Raman spectrum at every image pixel

  • Excellent lateral resolution

  • Outstanding depth resolution ideally suited for 3D image generation and depth profiles

  • Ultra-fast Raman imaging option with under one millisecond integration time per spectrum

  • Ultra-high throughput spectroscopic system for highest sensitivity and best performance in spectral resolution

  • Non-destructive imaging technique: no staining of fixation of the sample required

Raman General Operation Modes

  • Raman spectral imaging: acquisition of a complete Raman spectra at every image pixel
  • Planar (x-y-direction) and depth scans (z-direction) with manual sample positioning
  • Image stacks: 3D confocal Raman imaging
  • Time series
  • Single point Raman spectrum acquisition
  • Single-point depth profiling
  • Fibre-coupled UHTS spectrometer specifically designed for Raman microsopy and applications with low light intensities
  • Confocal Fluorescence Microscopy
  • Bright Field Microscopy
  • Additional lasers, several wavelenghts eligible
  • Additional UHTS-spectrometers (UV, VIS, NIR)
  • Automated, motorized sample positioning and measuring with piezo-driven scan stages
  • Automated confocal Raman imaging
  • Automated multi-area and multi-point measurements
  • Full automation available: see alpha300 apyron
  • Ultrafast Raman imaging, optional available
  • Upgradable for epi-fluorescence applications
  • Adapter for higher samples
  • TrueSurface for Raman depth profiling
  • Autofocus
  • Dark Field Microscopy, Phase Contrast Microscopy, and DIC optional

alpha300R stress in diamond

Confocal Raman image of stress in a diamond film. Color coded from red (compressive strain) to blue (tensile strain).

 

WITec 3D Raman image pollen honey

3D Raman Image of a pollen in crystalline honey. Image parameters: 150 x 150 x 50 pixels = 1,125,000

Raman spectra, scan area: 50 µm x 50 µm x 50µm, integration time per spectrum: 12.2 milliseconds, total acquisition time: 3h 50 min.

 

alpha300R 3D semiconductor depth scan

Color-coded Raman depth-scan of a strcutured GaN layer used for semiconductors. Violet: GaN; Green: fluorescence along the edges of the structure; Red: Stress in material.

 

alpha300R 3D emulsion

3D confocal color-coded Raman image of an emulsion of oil (green), alkane (magenta), and water (blue).

30 µm x 30 µm x 11.5 µm, 150 x 150 x 23 pixels, 517,500 Raman spectra, total acquisition time: 23 min.

  • Life Sciences 
  • Pharmaceutics & Cosmetics
  • Materials Science
  • Geoscience
  • Archeology & Arts v.v..

Click here to see more about RAMAN Series microscope's applications

 

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