alpha300 Ri – Inverted Raman Imaging Microscope
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alpha300 Ri – Inverted Raman Imaging Microscope
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alpha300 Ri – Inverted Raman Imaging Microscope

Raman confocal imaging is an analytical device equipped with modern technology and comprehensive analytical capabilities without affecting the sample. It uses the Raman effect, which is based on the inelastic scattering of light excited by molecules. A molecule's ability to interact with photons causes vibrations that disturb these chemical bonds, leading to specific energy shifts in the scattered light that can be seen in the sample's spectrum . Any chemical compound can produce a specific Raman spectrum when impacted or stimulated and can be easily identified by fingerprinting. The distribution of the components of a sample can be visualized as a general image of the Raman spectrum. Many versions and upgrades developed for the WITec alpha300 series are compatible with the Ri version. Other techniques related to inverted microscopy, such as fluorescence microscopy, are simultaneously integrated for easy image acquisition.

  • An inverted beam allows liquid samples to be placed on a fixed plane containing the sample suitable for quick and accurate measurements.
  • The sample platform on the Alpha300 Ri product line allows the study of even large, bulky and difficult-to-manipulate specimens.
  • The motor moves the sample, the sample platform allows for placing a number of accessories and support equipment during the research process.

• Non-destructive, contact-free, label-free chemical characterization

• True confocality, ideally suited to 3D image generation

• Lateral resolution limited only by physical law

• Consistent, repeatable positioning of liquid samples

• Bulky sample accommodation

• alpha300 series modularity for expandability and upgradeability

• Compatible with other microscopy techniques including: fluorescence, differential interference contrast (DIC) and phase-contrast

Raman General Operation Modes

  • Raman spectral imaging: Acquisition of complete hyperspectral Raman imaging data sets while continuously scanning.
  • Planar (x-y-direction) and depth scans (z-direction) with motorized sample positioning
  • Image stacks: 3D confocal Raman imaging
  • Fast and slow time series
  • Single point Raman spectrum acquisition and depth profiling
  • Fibre-coupled UHTS spectrometers specifically designed for Raman microsopy and applications with low light intensities
  • Confocal Fluorescence Microscopy

Basic Microscope Features

  • Research grade inverted optical microscope with 6x objective turret
  • Video system: Video CCD camera and/or fluorescence camera
  • LED white-light source for Köhler illumination
  • Binocular
  • Condensor lens for up to 7 contrasts (e. g. brightfield, DIC, Phase contrast, NAMC, etc.)
  • Sample holder accommodating various standard sample formats (e. g. microscopy slides, Terasakiplates, 35/65 mm dishes, Nunc flasks, counting chambers)
  • Internal filter block revolver
  • Motorized sample positioning and scanning stage in x- and y-direction, large travel range

Raman Optional/Upgradable Operation modes

  • A broad range of lasers and wavelenghts (from UV to IR) eligible
  • Additional UHTS spectrometers (UV, VIS, NIR)
  • Ultrafast Raman imaging optional available
  • Upgradable for time correlated single photon counting / fluorescence lifetime imaging (FLIM)
  • Autofocus

Ultrahigh-throughput WITec UHTS Spectrometers

  • Various lens-based, excitation optimized spectrometers (UV, VIS or NIR) available, all specifically designed for Raman microsopy and applications with low light intensities
  • Fibre-coupled ultrahigh-throughput optical instruments
  • Superior peak shape conservation

Correlative Raman-Fluorescence microscopy image of DAPI-marked eukaryotic cell nuclei

Correlative Raman Fluorescence Microscopy. Left: Nuclei labeled with DAPI. Right: Nucleoli (green) and endoplasmic reticulum (red) are identified by their Raman spectra. The Raman and fluorescence images are overlaid.

 

 

microscopic microscopeelectron microscopic microscopeatomic force microscopeinverted microscopic microscopeRaman microscopeRaman imageranalytical equipmentlaboratory equipmentsurface research geologyearth scienceslife sciencesmaterials researchindustryelectronics & semiconductorsenergyfossilsnuclear
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