alpha300 apyron – Automated Raman Imaging Microscope
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alpha300 apyron – Automated Raman Imaging Microscope
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alpha300 apyron – Automated Raman Imaging Microscope

alpha300 apyron – automation to its greatest extent

Since its first release in 2015, the alpha300 apyron has been WITec’s high-end fully-automated Raman microscope. WITec’s apyron systems are always equipped with the most recent features and developments, especially with respect to automation and user comfort.

Extensive automation

  • simplifies operation of the instrument.

  • requires less human input.

  • reduces potential sources of error.

  • enhances reproducibility.

  • allows for completely remote-controlled use in enclosed environments (i.e. in a glove box).

The alpha300 apyron is the ideal Raman microscope for:

  • multi-user laboratories with varying requirements and levels of user experience.
  • industry facilities with recurring experimental situations and an emphasis on time-critical turnover.
  • Raman newcomers with advanced imaging requirements.
  • veteran Raman microscopists seeking the next performance benchmark.
  • researchers employing remote operation, such as in enclosed environments.

In the alpha300 apyron, the entire workflow from system calibration through Raman measurements to data analysis is fully automated and can be completely remote-controlled. You never have to touch the microscope – except to change your sample.

Setup

  • TrueCal executes pre-configured calibration routines.

  • Laser safety class 1 (or 1M) compliant.

  • AutoBeam concept for automatic and motorized beam path alignment includes:

    • Motorized 6-position objective turret: positions selected objective and compensates offsets

    • Motorized illumination selector: switches between all microscopy illumination options

    • Motorized laser coupler: delivers up to 6 laser wavelengths from UV to NIR

    • AutoBeam output coupler: automatically optimizes signal & resolution and selects spectrometers

    • Motorized calibration source: fast & automatic multi-point spectrometer calibration over the full spectral range

    • Motorized Köhler illumination apertures: facilitate focusing and optimize contrast and homogeneity

    • Motorized polarization modules: freely rotatable automated excitation polarizer and detection analyzer

Raman imaging

  • TruePower sets and determines absolute laser power with <0.1 mW accuracy.

  • Instrument (re-)positions sample automatically with motorized and piezo-driven scan stages.

  • Focus stabilization finds and actively maintains focus during the entire measurement.

  • Motorized objective turret includes precise offset-compensation – AFM/SNOM objective-compatible.

  • EasyLink handheld controller offers intuitive instrument operation.

Data processing and display

  • Suite SIX software wizard guides the user through the processing of Raman spectra, from background reduction through image generation.

  • TrueComponent Analysis: automatically locates the sample components in an image and differentiates their individual spectra.

  • TrueMatch software identifies molecules by comparing measured spectra with existing databases.

Phase boundaries in a moisturizing shower gel

 

Emulsion shower gel + oil

Large-area Raman image and zoomed-in view of a moisturizing shower gel with increased oil content. Cyan and blue: aqueous phases; red: oil; yellow: emulsifier.

3D Raman imaging

 

D Raman image of a hand cream. Blue: water; red: oil; green: one of the hand cream’s moisturizing ingredients dissolved in oil. Scan range: 40 x 40 x 15 µm³

Low-frequency Raman imaging

LCysteine RayShield

Raman image of a solution of L-cysteine in a mixture of water and different herbal oils. Most of the L-cysteine is dissolved in the water phase (blue). In the oil phase (yellow), an undissolved cysteine crystal (red) is visible. The green areas likely represent one of the herbal oil ingredients. The Raman spectrum of L-cysteine has characteristic peaks in the low-frequency range, which were clearly resolved by the alpha300 apyron equipped with the WITec RayShield coupler.

Banana pulp

 

Banana Pulp Overlay+Spectra

Overlaid white-light and confocal Raman images of squashed banana pulp.

This high-resolution Raman image consists of more than a million spectra and was acquired in only 36 minutes.

Carotenoids (red), starch (green) and water (blue) were the most prominent ingredients.

Correlative Raman-AFM imaging

PTB Raman Head

White-light image (left), AFM image (middle) and Raman image (right) of a portrait of C. V. Raman on a silicon chip. The image is part of a calibration standard for Raman microscopes, produced and provided courtesy of the Physikalisch-Technische Bundesanstalt (PTB) in Braunschweig, Germany.

Automatic RaMan microscope alpha300 apyronRaman microscopealpha300 apyronraman spectroscopy
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